Microbubble fermentation of Trichoderma reesei for cellulase production ???券?敺格部?潮隞亦??Ｙ?蝬剝蝝??弦 A laboratory-scale microbubble dispersion (MBD) generator was shown to improve oxygen transfer to aerobic microorganisms when connected to a conventional air sparger. 撖阡?摰斤?敺格部??函????澆蝯望瘞??
this process setup had not been tested with fungi
where the morphology of microorganisms causes a very viscous broth rheology that impedes the oxygen mass transfer. ?嗉迨蝔?撠?函???鋡恍?霅? 銝???澆耦??靽頨急熄瘨脤?撣賊????瘞扳除?唾撓. Trichoderma reesei RUT C30 was chosen as a model fungus as it shows both the challenging rheology and a cellulase enzyme complex product that is shear-sensitive. 撖阡?銝剝?券?瘞?UT C30?
??嗆偌皞嗆雯???找??嗥?蝬剝蝝撠??銋??? Conventional and MBD sparged fermentations where carried out to compare the effect of aeration methods on oxygen transfer and product formation. MBD sparged fermentations showed fivefold higher kLa values and nearly twofold higher cell mass productivities. The cellulase enzyme activities were similar for both the MBD and the conventional sparged fermenters.?撖阡?隞亙蝯勗?蝬凝瘜∪?????潮瘜脰?
銝行?頛瘞扳除?唾撓???. 敺格部??潮銋La?箏蝯曹?5??蝝啗?鞈芷????? ?拇撘??蝥雁?萇?銋暑?批??詨榆銝之.